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| Urashima, Tadasu; Nakamura, Tadashi; Ikeda, Akiko; Asakuma, Sadaki; Arai, Ikichi; Saito, Tadao; Oftedal, Olav T.; 浦島, 匡; 中村, 正. |
| Carbohydrates were extracted from a sample of milk from a mink, Mustela vison (Family Mustelidae). Free neutral and acidic oligosaccharides were isolated from the carbohydrate fraction and their chemical structures were compared with those of white-nosed coati (Nasua narica, Procyonidae) and harbour seal (Phoca vitulina, Phocidae) that we had studied previously. The ratio of free lactose to milk oligosaccharides was similar to that in milk of the white-nosed coati; in both species, this ratio was much lower than that in the milk of most eutherians. The neutral oligosaccharides of mink milk had alpha(1-3)-linked Gal or alpha(1-2)-linked Fuc residues at their non-reducing ends, as in the neutral oligosaccharides of white-nosed coati milk. Some of the neutral... |
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Palavras-chave: Mink; Mustela vison; Mustelidae; Milk oligosaccharides; Neutral oligosaccharides; Sialyl oligosaccharides. |
| Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/729 |
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| Mori, Fumiki; Nakamura, Tadashi; Nakamura Osawa, Aya; Arai, Ikichi; Urashima, Tadasu; 森, 史樹; 中村, 正; 男澤－中村, 綾; 荒井, 威吉; 浦島, 匡. |
| Cell wall polysaccharides were separated from Leuconostoc mesenteroides subsp. cremoris isolated from “Filmjolk"，a fermented milk made commercially in Sweden. Two Components(CNPI and CNP2) were further purified by gelfiltration on HPLC and the molecu1ar weights were determined to be 850，000 and 81，000. CNP-2 was shown to contain two polysaccharides in major and minor by an lH-NMR. The major polysaccharide was shown to have a heptasaccharide repeating unit which contained five α and two β anomers，and also contained non reducing galactopyranose，O-2，3 disubstituted glucopyranose，O-3 substituted galactopyranose and O-3，6 disubstituted galactopyranose as units in a molar ratio of 3:1:1:2. On the other hand，the minor polysaccharide was shown to have a... |
| Ano: 2000 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2921 |
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| Urashima, Tadasu; Kobayashi, Mami; Asakuma, Sadaki; Uemura, Yusuke; Arai, Ikichi; Fukuda, Kenji; Saito, Tadao; Mogoe, Toshihiro; Ishikawa, Hajime; Fukui, Yutaka. |
| Samples of milk from a Bryde's whale and a Sei whale contained 2.7 g/100 mL and 1.7 g/100 mL of hexose, respectively. Both contained lactose as the dominant saccharide along with small amounts of Neu5Ac(alpha 2-3)Gal(beta 1-4)Glc(3'-N-acetylneuraminyllactose), Neu5Ac(alpha 2-6)Gal(beta 1-4) Glc(6-N- acetylneuraminyllactose) and Neu5Ac(alpha 2-6)Gal(beta 1-4)GlcNAc(beta 1-3)Gal(beta 1-4)Glc (LST c). The dominance of lactose in the carbohydrate of these milks is similar to that of Minke whale milk and bottlenose dolphin colostrum, but the oligosaccharide patterns are different from those of these two species, illustrating the heterogeneity of milk oligosaccharides among the Cetacea. (c) 2006 Elsevier Inc. All rights reserved. |
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Palavras-chave: Bryde's whale; Sei whale; Milk oligosaccharides; Balaenoptera edeni; Balaenoptera borealis lesson; Cetacea. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1659 |
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| Leo, Fiame; Hashida, Shukichi; Kumagai, Daiju; Uchida, Kenji; Motoshima, Hidemasa; Arai, Ikichi; Asakuma, Sadaki; Fukuda, Kenji; Urashima, Tadasu; 福田, 健二; 浦島, 匡. |
| A neutral exopolysaccharide produced in large amounts by Lactobacillus fermentum TDS030603 was purified from culture supernatants and its structure determined by monosaccharide analysis, methylation analysis and 1H-NMR spectroscopy. Optimum conditions for the production of this exopolysaccharide by the above bacterial strain, when cultured using cheese whey medium or MRS broth, were also investigated. The exopolysaccharide was found to be composed of D-galactose and D-glucose in the molar ratio of 1:2.5 and to consist of one major as well as one minor saccharide, both of which contained a tetrasaccharide repeating unit consisting of two α and two β anomers. The major saccharide contained non-reducing glucopyranose, O-3 substituted glucopyranose, O-6... |
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Palavras-chave: Lactic acid bacteria; Exopolysaccharide; Lactobacillus fermentum TDS030603. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4183 |
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| Sugai, Michiko; Takakuwa, N; Ohnishi, Masao; Arai, Ikichi; Urashima, Tadasu; Oda, Yuji. |
| From 2150 isolates from raw milk and milk products, yeast strains were surveyed to produce glucosylceramide from cheese whey. Most of the 54 strains that had accumulated a detectable amount of glucosylceramide were identified as Kluyveromyces lactis var. lactis. The cells of K. lactis var. lactis strain M-11 derived from domestic raw milk accumulated glucosylceramide 2.5-fold higher than K. lactis var. lactis NBRC 1267, the reference strain selected from the culture collections. Strain M-16 of K. lactis var. lactis derived from the same origin was found to synthesize a considerable amount of steryl glucoside in addition to glucosylceramide. Sequence analysis of ribosomal DNA intergenic spacer two regions revealed that strains M-11 and M-16 were diverged... |
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Palavras-chave: Glucosylceramide; Steryl glucoside; Cheese whey; Kluyveromyces lactis var; Lactis. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1055 |
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| Sumiyoshi, Wataru; Urashima, Tadasu; Nakamura, Tadashi; Arai, Ikichi; Nagasawa, Takashi; Saito, Tadao; Tsumura, Norihiko; Wang, Bing; Brand-Miller, Janette; Watanabe, Yoko; Kimura, Kazumasa; 住吉, 渉; 浦島, 匡; 中村, 正; 荒井, 威吉; 長澤, 孝志; 齋藤, 忠夫; 津村, 宣彦; 渡邉, 陽子; 木村, 一雅. |
| The concentrations of the six major sialyl oligosaccharides found in the milk of 24 Japanese women were determined using reverse phase high performance liquid chromatography of the 3-methyl-1-phenyl-5- pyrazolone derivatives of the oligosaccharides. The milk was collected at 4, 10, 30 and 100 days postpartum. The concentrations of all six oligosaccharides decreased during the course of lactation. The concentrations of 6'-sialyllactose, sialyllacto-N-tetraose b and disialyllacto-N-tetraose decreased only after 10 days postpartum, whereas those of sialyllacto-N-tetraose a and sialyllacto-N-neotetraose c as well as 3'-sialyllactose decreased from 4 days postpartum. Large differences were observed between present data and those previously reported for Italian... |
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Palavras-chave: Human milk; Sialyl oligosaccharide; Lactation period; PMP; Reverse phase HPLC. |
| Ano: 2003 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3287 |
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| 荒井, 威吉; 中島, 景典; 丸山, 千弘玲; 中村, 正; 戸羽, 隆宏; 浦島, 匡; Arai, Ikichi; Nakajima, Keisuke; Maruyama, Chigure; Nakamura, Tadashi; Toba, Takahiro; Urashima, Tadasu. |
| Microflora of lactic acid bacteria and yeasts of Laban which had been made with bovine or ovine milk by traditional natural fermentation in Yemen，were studied. The mean of viable cell counts of microorganisms in Laban was 6.4 x10⁸ cfu/ml. The 60 strains of lactic acid bacteria and 24 strains of yeasts were isolated and identified. The percentages of strains on lactic acid bacteria flora were as follows: Lactococcus lactis subsp.lactis① was dominant in 88%. Lc. lactis subsp. lactis②，Leuconostoc mesenteroides subsp. mesenteroides，and Leuc. lactis were in 5，5，and 2%，respectively. It was recognized that the some characteristics of Lc. lactis subsp. lactis① of the dominant strain were different from those of Lc. lactis subsp.lactis②. The percentages of strains... |
| Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2923 |
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| Nozaki, Hirofumi; Miyamoto, Akio; Hayashi, Ken-Go; Matsui, Motozumi; Yoshida, Takashi; Nakamura, Tadashi; Arai, Ikichi; Urashima, Tadasu; 宮本, 明夫; 松井, 基純; 中村, 正; 浦島, 匡. |
| The activity of α3-D-mannoside-β-1,2-N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was detected in bovine follicular fluid (bFF). The GnT I activity in bFF had a pH optimum of 5.8 and an absolute requirement for either Co2+, Mn2+, or Mg2+, the activity being stimulated by these cations in the above order. The apparent Km value for α1-3α1-6 mannopentaose of GnT I in bFF was 2.17 mM. The substrate specificity for the GnT I activity decreased in the following order: α1-3α1-6 mannopentaose> α1-3α1-6 mannotriose>α1-3 mannobiose. The GnT I activity in bFF from large atretic follicles was significantly higher than in that from large... |
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Palavras-chave: N-acetylglucosaminyltransferase I; Dominant follicle; Atretic follicle; Follicular fluid; Bovine. |
| Ano: 2004 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4185 |
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| 中村, 正; 菅井, 理子; 男澤, 綾; 有賀, 秀子; 小疇, 浩; キュラ, キーユキア; 荒井, 威吉; 浦島, 匡; Nakamura, Tadashi; Sugai, Michiko; Nakamura, Aya; Ariga, Hideko; Koaze, Hiroshi; Kiiyukia, Cura; Arai, Ikichi; Urashima, Tadasu. |
| Ano: 1999 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1877 |
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